Next Generation Plant Ecology & Evolution workshop 2011

NGS logo

18 - 19th October 2011, Royal Botanic Garden Edinburgh


We would like to express out thanks to all the delegates who participated in the workshop and helped make the event a success. Two of the presentations from the introductory session are now available to download: Urmi Trivedi - Next generation sequencing (NGS) Bioinformatics - Challenges and strategies, and Karim Gharbi NGS Technologies a user’s guide. See below, there is also a group photograph from the workshop.

A two day workshop on how next generation sequencing technologies can transform research in plant ecology and evolution. Sessions include how to use NGS to:

  • Estimate gene flow and population level genetic differentiation (Leader: Rémy Petit, UMR Biodiversité Gènes et Communautés)
  • Understand adaptation and fitness (Leader: Michael Arnold, University of Georgia)
  • Sample diversity of environmental samples (Leader: Eric Coissac, Université Joseph Fourier)
  • Obtain the genes for a specified phenotype (Leader: Ian Baldwin, Max Planck Institute)
  • Generate more informative phylogenetic markers (Leader: Mark Chase, Royal Botanic Garden Kew)
  • Understand the genetic changes of speciation (Leader: Pam Soltis, University of Florida)

Keynote speakers:

  • Christian Lexer (University of Fribourg)
  • Douglas Soltis (University of Florida)

This is an excellent opportunity for plant researchers to learn about exciting new applications of next generation sequencing technologies.


The Royal Botanic Garden Edinburgh is a world-renowned scientific centre for the study of plants, their diversity and conservation. The Garden is situated 2.3 Km (1.4 miles) to the north of the city centre.


Monday 17th October
You may be interested to attend a Botanical Society of Scotland Public Lecture:
Hybrid fitness, adaptation and evolutionary diversification: lessons learned from the Louisiana
Irises by Michael Arnold. RBGE Lecture Theatre 17:30 (tea available from 17:00)

Tuesday 18th October

Registration 08:00-10:00 coffee available from 09:00

Opening Session 10:00-12:15 (Chair: Alex Twyford)
10:00 Welcome to the RBGE by Mary Gibby (RBGE director of Science)
10:05 Welcome to the workshop by Alex Twyford
10:15 Next-Generation Sequencing Technologies: a User’s Guide by Karim Gharbi
10:45 Next Generation Sequencing Bioinformatics – Challenges and Strategies by Urmi Trivedi

Keynote Talk
11:15 The use of next generation sequencing in the study of non-model organisms by Douglas Soltis

Lunch 12:15-13:45
Optional herbarium tour or living collection tour, departs at 1245, maximum 20 people each

‘How-to’ Session 1 13:45-15:15 (Chair: David Burslem)
13:45 Next-generation studies of gene flow and genetic differentiation by Rémy Petit
14:30 The Genomics of Adaptation and Fitness: Christmas Past, Present and Future by Michael Arnold

Afternoon coffee 15:15-15:45

‘How-to’ Session 2 15:45-18:00 (Chair: Catherine Kidner)
15:45 Manipulation of gene expression remains the gold standard for the functional analyses of genes: will plant ecologists and evolutionary biologists fully join the genomics revolution? by Ian Baldwin
16:30 New generation sequencing: an aid to developing new more-variable phylogenetic markers by Mark Chase
17:15 Next-Generation Sequencing and the Analysis of Reticulate Speciation by Pam Soltis

Posters and drinks reception, 18:00-19:00 in the Fletcher Building
Conference meal, 1900-2200 in the Conference Room

Wednesday 19th October

‘How to’ session 3 09:00-10:30 (Chair: Richard Buggs)
09:00 DNA Metabarcoding: High-throughput biodiversity assessment by Eric Coissac
09:45 RAD-seq, a cost-effective method for SNP discovery and genotyping using next-generation sequencing by Pablo Fuentes-Utrilla

Morning coffee 1030-1110

Keynote Talk
11:10 Next generation ecology & evolution: towards tracking the genomic signatures of diversification by Christian Lexer

Molecular Ecology Prize presentation and group photo

Lunch 12:30-14:00
Optional herbarium tour or living collection tour, departs at 1300, maximum 20 people

Research presentations session 1 14:00-15:20 (Chair: Peter Hollingsworth)
14:00 Cost-effective method for the development of microsatellite loci combining multiplex microsatellite enrichment, next-generation sequencing, and the use of
universal fluorescently-labelled primers by Claire Micheneau
14:20 Single-copy conserved orthologous genes: promises and prospects for the application of next generation sequencing technologies in tropical species? by Olivier

14:40 Genomic approaches to understanding mating system and ploidy evolution in Arabidopsis lyrata by Barbara Mable
15:00 Exploring Giant Plant Genomes with Next-Generation Sequencing by Laura Kelly

Afternoon coffee 15:20-16:00

Research presentations session 2. 16:00-17:10 (Chair: Richard Ennos)
16:00 Comparative transcriptomics and genomics: determining the regulators of petal spot development and the evolution of this specialised floral trait within the species
complex Gorteria diffusa by Rachel Walker
14:20 Plant Y-chromosome degeneration is retarded by haploid purifying selection by Dmitry Filatov
14:40 A coastal cline in sodium accumulation in Arabidopsis thaliana is driven by natural variation of the sodium transporter AtHKT1;1 by David Salt
Thanks and Close 17:10 by Alex Twyford

Student Prize

We are delighted to announce that Rachel Walker has been awarded the main student prize to attend the workshop, based on an excellent abstract. Due to the high quality of abstracts received, three additional students have been awarded runner-up prizes: Setareh Mohammadin, Heidi Hirsch and Britta Kümpers. Thanks to everyone who entered.

Information for contributors: see here.

The NGS 2011 Workshop Delegates:

NGS Delegates
For a larger image click on the one above.

Organizing committee:

Alex Twyford (Royal Botanic Garden Edinburgh, University of Edinburgh).
Richard Buggs (Queen Mary, University of London).
David Burslem (University of Aberdeen).
Richard Ennos (University of Edinburgh).
Peter Hollingsworth (Royal Botanic Garden Edinburgh).
Catherine Kidner (Royal Botanic Garden Edinburgh, University of Edinburgh).


Image credits: Group photograph - Lynsey Wilson (RBGE), Workshop logo - Chris Jeffree and Catherine Kidner.



This meeting was held in association with:

Bot Soc logo Genetic Soc logo BES logo
Kappa Bioscience logo anachem logo VWR International logo
Bioline logo agilent logo Roche logo




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